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fc receptor blocking antibody 2 4g2  (Bio X Cell)


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    Structured Review

    Bio X Cell fc receptor blocking antibody 2 4g2

    Fc Receptor Blocking Antibody 2 4g2, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+receptor+blocking+antibody+2+4g2/InVivoMAb+anti-mouse+CD16+CD32/pmc09451539-428-20-24
    Average 96 stars, based on 370 article reviews
    fc receptor blocking antibody 2 4g2 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Epigenetic remodeling by vitamin C potentiates plasma cell differentiation"

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation

    Journal: eLife

    doi: 10.7554/eLife.73754


    Figure Legend Snippet:

    Techniques Used: Dot Blot, Enzyme-linked Immunosorbent Assay, Purification, Extraction, Saline, Flow Cytometry, Ligation, Sequencing, Cell Isolation, Isolation, Multiplex Assay, Recombinant, Software

    Related Articles

    FACS:

    Article Title: Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells
    Article Snippet: .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (Biolegend) at RT for 30 mins. .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (Biolegend) at RT for 30 mins.

    Article Title: Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells
    Article Snippet: To induce plasma cell differentiation, cells were cultured with recombinant human IL-21 (100 ng/mL; Peprotech) and either with: 0.01% PANSORBIN (Millipore); or ODN (2.5 μM; Invivogen, San Diego, CA) and anti-hCD40 (1 μg/mL; BioLegend). .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice. .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice.

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation
    Article Snippet: .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (STEMCELL Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (BioLegend) at RT for 30 min. .. Cells were washed twice with FACS buffer, fixed with 1% paraformaldehyde (Thermo Fisher), and analyzed by FACS.

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation
    Article Snippet: .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1 mM ethylenediaminetetraacetic acid [EDTA], 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 min on ice. ..

    Blocking Assay:

    Article Title: Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells
    Article Snippet: .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (Biolegend) at RT for 30 mins. .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (Stemcell Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (Biolegend) at RT for 30 mins.

    Article Title: Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells
    Article Snippet: To induce plasma cell differentiation, cells were cultured with recombinant human IL-21 (100 ng/mL; Peprotech) and either with: 0.01% PANSORBIN (Millipore); or ODN (2.5 μM; Invivogen, San Diego, CA) and anti-hCD40 (1 μg/mL; BioLegend). .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice. .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice.

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation
    Article Snippet: .. The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (STEMCELL Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (BioLegend) at RT for 30 min. .. Cells were washed twice with FACS buffer, fixed with 1% paraformaldehyde (Thermo Fisher), and analyzed by FACS.

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation
    Article Snippet: .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1 mM ethylenediaminetetraacetic acid [EDTA], 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 min on ice. ..

    Incubation:

    Article Title: Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells
    Article Snippet: To induce plasma cell differentiation, cells were cultured with recombinant human IL-21 (100 ng/mL; Peprotech) and either with: 0.01% PANSORBIN (Millipore); or ODN (2.5 μM; Invivogen, San Diego, CA) and anti-hCD40 (1 μg/mL; BioLegend). .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice. .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1mM Ethylenediaminetetraacetic acid/EDTA, 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 mins on ice.

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation
    Article Snippet: .. Cells were resuspended in FACS buffer (1% bovine serum albumin, 1 mM ethylenediaminetetraacetic acid [EDTA], 0.05% sodium azide) and incubated with Fc-receptor blocking antibody 2.4G2 (5 μg/mL; BioXCell, Lebanon, NH) for at least 10 min on ice. ..



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    Image Search Results


    Journal: eLife

    Article Title: Epigenetic remodeling by vitamin C potentiates plasma cell differentiation

    doi: 10.7554/eLife.73754

    Figure Lengend Snippet:

    Article Snippet: The next day, cells were washed at least twice with FACS buffer to remove the fixative before incubating with the Fc-receptor blocking antibody 2.4G2 (BioXCell) and rat serum (STEMCELL Technologies), followed by the anti-phosphoSTAT3 (Tyr705) antibody (BioLegend) at RT for 30 min.

    Techniques: Dot Blot, Enzyme-linked Immunosorbent Assay, Purification, Extraction, Saline, Flow Cytometry, Ligation, Sequencing, Cell Isolation, Isolation, Multiplex Assay, Recombinant, Software